Zero wine is an open source (GPL v2) research project to dynamically analyze the behavior of malware. Zero wine just runs the malware using WINE in a safe virtual sandbox (in an isolated environment) collecting information about the APIs called by the program.
The output generated by wine (using the debug environment variable WINEDEBUG) are the API calls used by the malware (and the values used by it, of course). With this information, analyzing malware's behavior turns out to be very easy.
Zero wine is distributed as one QEMU virtual machine image with a Debian operating system installed. The image contains software to upload and analyze malware and to generate reports based on the information gathered (this software is stored in /home/malware/zerowine).
Running the distributed virtual machine with the correct command line options (use the supplied startup shell script to run the virtual machine) provides a web based (web server is written in python) graphical interface to upload malware to be analyzed (a CGI written, also, in python).
When a new malware is uploaded, it is copied to the directory /tmp/vir/MD5_OF_THE_FILE, then, the previous created WINE environment (WINEPREFIX if you prefer) is removed and a backup system is untared (the backup system is /home/malware/backup/backup.tar.gz). After this operation, the malware is executed using the shell script malware_launcher.sh (the file is stored in the folder /home/malware/bin).
For more details please refer here.